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. Author manuscript; available in PMC: 2019 Feb 3.
Published in final edited form as: Biochim Biophys Acta. 2018 Feb 3;1861(2):72–79. doi: 10.1016/j.bbagrm.2018.01.002

Figure 5.

Figure 5

Analysis of total H3 abundance across the ATF3 gene locus during activation of the AAR and UPR pathways. (A) Schematic showing ATF3 gene structure and primer locations for ChIP analysis at specific regions of the gene locus. (B) HepG2 cells were cultured in DMEM ± 2.5 mM HisOH or ± 50 nM Tg for 4 h or 16 h. The association of total histone H3, a reflection of nucleosome abundance, was assessed by ChIP analysis. The data, plotted as the ratio to input DNA, are the means ± S.D. for at least three replicates. Each experiment was repeated to ensure reproducibility between batches of cells.