Figure 4.

(a) Sample images of GFP-Life-act DC inhibited with CK-666, showing GFP-labeled actin filaments (left) and bright field images of the same cell (right). Scale bar equals 20 μm. Quantification of WT DC chemokinesis in the presence of CK-666, an Arp2/3 inhibitor (b) average speed, (c) persistence length and (d) random motility coefficient (values taken from Figure 3 and included for reference). Three different CK-666 concentrations were used: from left to right 1 μM, 10 μM and 100 μM. Figures represent average values ± SEM, for > 290 DCs from at least three independent experiments per condition. Statistical significance calculated with single factor ANOVA and post hoc Tukey test. Indicates significant difference compared to WT DCs. *p<0.05, **p<0.01