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. Author manuscript; available in PMC: 2019 Feb 26.
Published in final edited form as: Dev Cell. 2018 Feb 26;44(4):433–446.e7. doi: 10.1016/j.devcel.2018.01.021

Figure 6. Increasing the proportion of polyploid cardiomyocytes impairs heart regeneration.

Figure 6

(A–C) Adjacent sections from 45 dpr hearts from the indicated cohorts, immunostained for Tropomyosin and GFP (left) or stained with AFOG (right). n = 2 (0), 5 (5), 9 (0) hearts (number of hearts that showed complete regeneration indicated within the brackets), respectively. (D) Quantification of the scar area of hearts from (B,C) normalized to the ventricular area (n= 5, 9 hearts; solid black line indicates the mean); ***P<0.001, two-tailed unpaired t-test. (E–G) Sections from 14 dpr hearts of the indicated cohorts, immunostained for Mef2 and BrdU, as described in Figure 5G–5H. White arrows indicate BrdU+ cardiomyocyte nuclei. (H) Cardiomyocyte BrdU labeling index in injury sites in experiments from (E–G). Box-and-whisker plot. n = 11, 10, 7 ventricles, respectively. ****P<0.0001; **P<0.01 by one-way ANOVA followed by Tukey’s multiple comparisons test). See also Figures S7 and S8. CM, cardiomyocyte; ER, early recombination; HS, heat-shocked. Scale bars: 50 μm.