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Microtissues cultured in suspension aggregated to form larger tissue structures. A, B) By day 7, neovessels were evident in tissue masses. C, D) By day 14, networks of vessels had formed in tissue masses made with 1:3 MVEC:FB microtissues, but were less evident in those made at 1:1. E) Fluorescence staining and F) PECAM/CD-31 IHC confirmed that vessels within tissue structures were created by MVEC.
