Table 1.
Details of antibodies showing the source, catalog number, dilution, host species and antigen sequences.
S.No | Peptide/protein target | Antigen sequence | Name of Antibody | Manufacturer; catalog # | Antibody type | Dilution used |
---|---|---|---|---|---|---|
1 | BCL2 | N terminal amino acids 1–18 of Human Bcl-2; AGRTGYDNREIVMKYIHY | Rabbit Anti-Bcl-2 | Abcam; ab7973 | Rabbit polyclonal | 1:300 |
2 | BCL-XL | Synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL | Rabbit Anti-BCL-xl | Cell Signaling; 2762 | Rabbit polyclonal | 1:50 |
3 | XIAP | Synthetic peptide corresponding to a region surrounding serine 245 of human XIAP | Rabbit Anti-XIAP | Cell Signaling; 2045 | Rabbit monoclonal | 1:100 |
4 | BAX | Synthesized peptide derived from human BAX | Rabbit Anti-BAX | Sigma; SAB4502549 | Rabbit polyclonal | 1:1500 |
5 | AIF | Purified recombinant fragment of Human AIF expressed in E. Coli | Mouse Anti-AIF | Abcam; Ab119917 | Mouse monoclonal | 1:500 |
6 | Cleaved- Caspase-3 | Synthetic peptide corresponding to amino-terminal residues adjacent to (Asp175) in human caspase-3 | Rabbit Anti-Cleaved- Caspase-3 | Cell Signaling; 9661 | Rabbit polyclonal | 1:100 |
7 | ATM | Recombinant fragment, corresponding to amino acids 1980–2338 of Human ATM | Rabbit Anti-ATM | Abcam; Ab10941 | Rabbit polyclonal | 1:400 |
8 | P53 | Synthetic peptide within Human p53 aa 13–17 (PLSQE) conjugated to Keyhole Limpet Haemocyanin (KLH). | Rabbit Anti-P53 | Abcam; Ab131442 | Rabbit polyclonal | 1:2000 |
9 | PUMA | Synthetic peptide corresponding to C terminal amino acids 180–193 (PLPRGHR APEMEPN) of Human PUMA alpha | Rabbit Anti-PUMA | Abcam; Ab9643 | Rabbit polyclonal | 1:2000 |
10 | NOXA | Synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa | Rabbit Anti-Noxa | Abcam; Ab36833 | Rabbit polyclonal | 1:1000 |
11 | P27 | Synthetic peptide corresponding to the sequence of mouse p27 | Rabbit Anti-P27 | Cell Signaling; 2552 | Rabbit polyclonal | 1:50 |