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. Author manuscript; available in PMC: 2018 Oct 11.
Published in final edited form as: Cell Host Microbe. 2017 Sep 28;22(4):494–506.e8. doi: 10.1016/j.chom.2017.08.020

Figure 2.

Figure 2

Competition of single CPS-expressing strains in germ-free mice reveals distinct advantages conferred by specific CPS. Eight single CPS-expressing strains and an acapsular strain were pooled and inoculated into germ-free mice of 3 genotypes (n=5 mice/group): A, D) wild-type; B, E) MyD88−/−/Trif−/−; C, F) Rag1−/−. Panels A–C include a linear axis for relative abundance, whereas panels D–F use a logarithmic axis to reveal strains that have fallen below the limit of detection. All mice were fed a high-fiber diet. Relative abundance of each strain in stool was determined at regular intervals via qPCR. The relative abundance in the inoculum is represented as “Day 0”. Each mouse was individually caged for this experiment. Data are represented as mean ± SEM. Asterisks (in matching colors to bacterial strain) indicate significant differences (* p < .05, ** p < .01; Kruskal-Wallis test, with Benjamini-Hochberg correction) in relative strain abundance between wild-type mice and the mutant mouse genotype shown in that panel at each time point. See also Figures S2–6 and Table S3. LOD: Limit of detection.