Table 3. Effect of different concentrations of tunicamycin on the developmental potential of mouse embryos in vitro.
Treated group | Zygote No | 2 Cell | 4 Cell | 8 Cell | Blastocyst |
Control | 185 | 92.50 ± 3.27 a | 82.00 ± 5.83 a | 70.50 ± 3.53 a | 50.00 ± 3.16 a |
TM (0.25) | 190 | 90.00 ± 2.58 a | 78.50 ± 3.16 a | 65.50 ± 2.73 a | 44.50 ± 3.90 a |
TM (0.5) | 170 | 87.00 ± 4.16 a | 74.50 ± 5.74 a | 36.00 ± 3.14 b | 18.50 ± 1.30 b |
TM (0.75) | 180 | 85.50 ± 3.20 a | 73.00 ± 2.80 a | 26.00 ± 3.39 c | 10.00 ± 2.23 c |
Data are the proportions of zygotes reaching the indicated stages within each treatment. Data are expressed as a mean value ± standard error of the mean (SEM) of three independent experiments. Different letters in the same column mean significant difference between the treatments (P < 0.05).