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. 2017 Dec 21;103(3):406–416. doi: 10.3324/haematol.2017.177394

Figure 3.

Figure 3.

Apoptosis of miR-144/451−/− bone marrow and spleen erythroblasts is increased under other stress conditions. (A and B) Results of flow cytometric analysis of nucleated erythroid cell apoptosis in spleen (A) and bone marrow (B) after phenylhydrazine (PHZ) administration. miR-144/451−/− mice (n=5) and wild-type (WT) littermates (n=5) were treated with PHZ (120 mg/kg) on days 0 and 1. Splenocytes and bone marrow cells were harvested four days after PHZ injection. Ter119+Hoechst+ cells are nucleated erythroid cells. Annexin V+PI− near IR− cells are early apoptotic cells. Ter119− cells are non-erythroid cells. **P<0.01 (t-test). (C and D) Flow cytometric analysis of early apoptosis rates of spleen (C) and bone marrow (D) nucleated erythroblasts after bleeding. miR-144/451−/− mice (n=5) and WT littermates (n=5) had 0.4 mL of blood drawn on two consecutive days. Splenocytes and bone marrow cells were harvested on day 3. **P<0.01 (t-test). Note: more early apoptotic erythroblasts were seen in miR-144/451−/− mice.