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. 2018 Mar 1;5:9. doi: 10.1038/s41438-018-0017-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Quantitative real-time PCR analysis of the abundance of miR482b in WT, overexpressing (a) and silencing (b) tomato lines. c Detached-leaf inoculated with P. infestans showing disease signs (scale bars = 0.5 cm). Top: disease symptoms; middle: leaves were photographed in UV-light; bottom: trypan Blue staining for detection of dead cells. d The relative lesion diameter. e Whole-plant assay of P. infestans-infected tomato plants showing disease index of WT, overexpressing and silencing tomato lines. f Transcript accumulation of P. infestans Actin gene in these inoculated plants. g MDA content of the leaves from WT, and overexpressing and silencing tomato lines at 5 days after inoculation with P. infestans. Data are the means ± SEs from three independent experiments. Letters indicate significant difference among samples, and letters shared in common between or among the groups indicate no significant difference (P < 0.05)