Fig. 4.

The enhanced formation of nrMTs fails if the sequestering of Stu1 at an uaKT is defective and the spindle remains uncompromised. a–e The experiment with stu1∆CL and Mps1-depleted cells (genotypes see Supplementary Table 1) was performed as described in Fig. 2 and visualized as in Fig. 3b. b–e The number and length of nrMTs were determined. Cells with an uaKT were observed for 15 timepoints or until the uaKT was captured. The data corresponding to WT cells with an uaKT, as shown in Fig. 3d and e, has been included for comparison. Statistics, see Supplementary Table 3