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. 2018 Feb 28;9:872. doi: 10.1038/s41467-018-02873-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Long-term in vivo imaging of neural structure. Time-lapse two-photon imaging of the dendritic arbor (upper) and axonal boutons (lower) of a fluorescent mushroom body output neuron (MBON-α3) in a live fly (MB093C-GAL4>20×UAS-6×GFP). Major dendritic structures (yellow arrowheads) and axonal boutons (red arrowheads) were stable for 10 days. Scale bars: 10 μm. See also Supplementary Fig. 2 for additional time-lapse image datasets