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. 2018 Feb 28;9:875. doi: 10.1038/s41467-018-03256-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — : Auxin signalling is required in cambium stem cells. a–f Confocal analyses of stem bases from plants containing the auxin response markers pDR5rev:GFP (a–c) or pDR5revV2:YFP (d–f) and the stem cell marker pPXY:CFP. Overlapping foci between pPXY:CFP (red) and the respective auxin response marker activities are marked by arrows (c, f). Asterisks mark the vascular bundles. Size bars represent 100 µm. Propidium iodide (PI) staining in blue. g–l Toluidine blue stained cross sections at the stem base of wild type (g) pWOX4:AlcR; pAlcA:iaaM (h), pPXY:Myc-GR-bdl (i, j) and pBDL:Myc-GR-bdl (k, l) plants after long-term EtOH (g, h), mock (i, k) or Dex (j, l) treatment. Interfascicular regions are shown and interfascicular cambium-derived tissues (ICD) are marked (red bar). Size bars represent 50 µm. m Quantification of ICD tissue extension at the stem base of wild type, pWOX4:AlcR; palcA:iaaM (WOX4»iaaM), pPXY:Myc-GR-bdl and pBDL:Myc-GR-bdl plants after long-term EtOH (wild type and WOX4»iaaM), mock (grey) or Dex (yellow) treatment. Student’s T-test (pPXY:Myc-GR-bdl (line 2) p = 9.82E-06 and pBDL:Myc-GR-bdl p = 0.001 and p = 0.03) or Welch’s T-test (wild type and WOX4»iaaM p = 9.24E-09 and pPXY:Myc-GR-bdl (line 1) p = 5.2E-06) were performed comparing wild type and WOX4»iaaM and mock and Dex, respectively (Sample sizes n = 8–16). The T-bars that extend from the boxes (whiskers) are expected to include 95% of the data. Significance is indicated by asterisks