Table 1.

Pair-wise comparison of total free FAs in the medium produced by E. coli strains expressing pairs of TE mutants that differ only by the presence or absence of specific point mutation(s)

Point mutation(s)	Free FAs titer (µM)
	TEs without the mutation(s)		TEs with the mutation(s)
L257F	CvB2MT32	330.6	CvB2MT33	569.5
	CvB2MT22	84.6	CvB2MT29	287.1
	CvB2MT2	81.7	CvB2MT12	148.9
	CvB2MT27	230	CvB2MT31	393.1
	CvB2MT16	308	CvB2MT18	299.9
	CvB2MT10	170.9	CvB2MT15	149.6
	CvFatB2	175.2	CvB2MT4	199.1
	CvB2MT14	108.3	CvB2MT23	384.7
	Paired	t-test	p-value	0.012
I260L	CvB2MT31	393.1	CvB2MT33	569.5
	CvB2MT27	230	CvB2MT32	330.6
	CvB2MT10	170.9	CvB2MT16	308
	CvB2MT15	149.6	CvB2MT18	299.9
	CvB2MT3	90.3	CvB2MT13	183.1
	CvB2MT14	108.3	CvB2MT24	400.8
	CvFatB2	175.2	CvB2MT5	456.5
	CvB2MT21	201.2	CvB2MT28	328.3
	Paired	t-test	p-value	0.00022
A276P, D282N, L289F	CvFatB2	175.2	CvB2MT14	108.3
	CvB2MT6	31.1	CvB2MT17	113.7
	CvB2MT25	285.0	CvB2MT34	464.0
	CvB2MT18	299.9	CvB2MT33	569.5
	CvB2MT12	148.9	CvB2MT29	287.1
	CvB2MT16	308.0	CvB2MT32	330.6
	CvB2MT15	149.6	CvB2MT31	393.1
	CvB2MT10	170.9	CvB2MT27	230.0
	CvB2MT2	81.7	CvB2MT22	84.6
	CvB2MT4	199.1	CvB2MT23	384.7
	CvB2MT5	456.5	CvB2MT24	400.8
	CvB2MT13	183.0	CvB2MT28	328.3
	CvB2MT3	90.3	CvB2MT21	201.2
	Paired	t-test	p-value	0.0025