Skip to main content
. 2018 Feb 28;3(1):e00006-18. doi: 10.1128/mSphere.00006-18

TABLE 1 .

Strains, plasmids, and primers used in this study

Strain or
plasmid		 Genotype or description Source, derivation,
or reference(s)
Strains
    E. coli χ7213 K-12; thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 ΔasdA4 recA1 RP42-Tc::Mu [λpir] Kmr 60
    S. Typhi
        χ3769 Ty2; RpoS− 61
        χ8073 CVD908-htrA; ΔaroC1019 ΔaroD1013 ΔhtrA 15, 17
        χ8205 Ty21a; unknown; Vi− GalE− 13
        χ8444 Ty800; ΔphoPQ23 14
        χ9639 ΔPcrp527::TT araC PBAD crp ΔPfur81::TT araC PBAD fur Δpmi-2426 Δ(gmd-fcl)-26
ΔrelA198::araC PBAD lacI TT ΔaraE25 ΔtviABCDE10 ΔagfBAC811 ΔsopB1925 ΔasdA33 		16
        χ9640 ΔPcrp527::TT araC PBAD crp ΔPfur81::TT araC PBAD fur Δpmi-2426 Δ(gmd-fcl)-26
ΔrelA198::araC PBAD lacI TT ΔaraE25 ΔtviABCDE10 ΔagfBAC811 ΔsopB1925 ΔasdA33 RpoS+ 		16
        χ11498 Unknown; Vi− GalE− RpoS+ χ8205
        χ11499 ΔphoPQ23 RpoS+ χ8444
        χ11513 ΔaroC1019 ΔaroD1013 ΔhtrA RpoS+ χ8073
Plasmids
    pYA3467 Suicide vector containing rpoS from serotype Typhi ISP1820 used in generating RpoS+ strains 42
    pYA3493 pYA3342 derivative β-lactamase signal sequence-based periplasmic secretion plasmid 7