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. 2018 Feb 28;8:3817. doi: 10.1038/s41598-018-22010-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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mir-138-1 cKO sciatic nerves do not display alterations in proliferation, cell cycle exit, and in cell numbers. (A) EdU (green), EGR2 (red) and DAPI (blue) staining of P4 mutant and control sciatic nerves. (B) Quantification of EGR2 expression (EGR2+/DAPI+) and cell proliferation rate (EdU+/DAPI+) in P4, and quit fraction (Ki67-EdU+/EdU+) and cell numbers (DAPI+/area) in P4 mutant and control sciatic nerves. There is no significant difference detected in the number of EGR2-expressing cells (N ≥ 4; P4, p = 0.7847), cell proliferation rate (N ≥ 4; P4, p = 0.6942), quit fraction (N ≥ 4; P4, p = 0.1500), or cell number per unit area (N ≥ 4; P4, p = 0.4910). Black bar = controls. Gray bar = mir-138-1 cKOs.