Table 2.
Intermediate precision of optimized SEC
| Parameter | Mean ± uca | CV |
|---|---|---|
| Instrument Qualification (IQ) Standard | ||
| γ-globulin Migration Time (min) | 3.90 ± 0.08 | 2.0% |
| Ovalbumin Migration Time (min) | 4.69 ± 0.10 | 2.0% |
| PS 8670 | ||
| Monomeric Purity (%) | 98.78 ± 0.12 | 0.1% |
| HMW Relative Abundance (%) |
1.02 ± 0.12 | 11.6% |
| LMW Relative Abundance (%) |
0.20 ± 0.01 | 4.1. % |
| Monomer Retention Time (min) | 3.95 ± 0.08 | 2.1% |
| Resolution (Dimer-Monomer) | 1.95 ± 0.05 | 2.7. % |
aStated uncertainty represents the intermediate precision reported as a combined standard uncertainty, at a level of one standard deviation, based on ANOVA analysis as described in ESM (n = 24)