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. 2018 Feb 28;8:3797. doi: 10.1038/s41598-018-22218-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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iRhom2 deficiency does not affect intestinal injury after intestinal ischemia-reperfusion (I/R). (A) Representative histological sections of H&E-stained intestines from the wild-type (WT) and iRhom2 knockout (KO) mice are shown (x100). Scale bars: 100 μm. The intestinal injury was not different between the iRhom2 WT and KO mice. (B) The severity of the intestinal injury was assessed using a histological grading system based on the following criteria (score: 0–5): preservation of the villi, epithelial degeneration/necrosis, erosion or ulceration, and mucin depletion.