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. 2018 Feb 28;8:3821. doi: 10.1038/s41598-018-20957-2

Figure 1.

Figure 1

IL-23 secretion from DCs alone or in trans-well co-cultures with GSCs. (a) The level of IL-23 expression from DCs in trans-well co-cultures with GSCs of a non-cancer control individual (N1) and a cancer patient (T21, GSCs from cancer lesion; N21, GSCs from normal adjacent gastric tissue) in the absence or presence of Hp stimulation (Hp:MOI = 1:200) was analyzed by ELISA. Hp were added for 24 hrs in the upper chamber for stimulation of GSCs (2 × 105 cells/ml). (b) The level of IL-23 expression from DCs in direct contact E. coli-derived LPS (100 ng/ml) or Hp for 24 hrs were used as control. (c) IL-23 expression in the other three sets of matched cancer GSCs (T2, T6, and T9) and adjacent normal GSCs (N2, N6, N9) obtained from gastric cancer patients. Hp-GSCs-conditioned DCs regulated IL-17 expression from CD4+ T cells. Level of IL-17 expression in CD4+ T cells co-cultured with conditioned DCs at a 1:5 (DC/CD4+ T) ratio was evaluated at day 3 (d) or day 5 (e). (f) IL-17 expression in three cancer GSCs (T2, T9, and T21) obtained from gastric cancer patients in the absence or presence of Hp stimulation (Hp:MOI = 1:200). (g) IL-17 production was measured in the presence or absence of IL-23-neutralizing Abs. The results are expressed as means ± SE, and the data shown represent independent experiments from three-five different DC donors. Mann- Whitney U test was used and for pair-wise comparisons only. *p < 0.05, **p < 0.01, ***p < 0.001.