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. 2018 Feb 28;8:3821. doi: 10.1038/s41598-018-20957-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Soluble factor PGE₂ secreted from Hp treated GSCs. (a) Plating 2 × 10⁵ GSCs cells/well with or without Hp infection (MOI = 1:200) for 24 hrs. (b) Using transwell co-culture experiment, plating 2 × 10⁵ GSCs cells/well with Hp infection (MOI = 1:200) in the apical side of transwell insert with a total of 2 × 10⁵ DCs were cultured in basolateral side of 24-well plates for 24 hrs. Supernatants were collected for PGE₂ analysis by ELISA. GSCs N1 derived from a Hp negative, non-cancer control subject was used for reference. Paired GSCs of tumor (T2, T6, T9, and T21) and adjacent normal part (N2, N6, N9, N21) were obtained from gastric cancer patients. PGE₂ data are expressed as mean ± SE of three-five independent experiments from different DC donors. Mann-Whitney U-test was used and for pair-wise comparisons only. *p < 0.05, **p < 0.01, ***p < 0.001.