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. 2018 Feb 28;8:3821. doi: 10.1038/s41598-018-20957-2

Figure 5.

Figure 5

Immunofluorescence analysis of COLEC12 expression in (a) GSCs primary cell culture from various sources as indicated. GSCs were co-stained with rabit anti-COLEC12 primary Abs/Alexa 488 goat anti-rabbit secondary Abs (Green) and DAPI (Blue, for staining nucleus). An isotype control Ab was included as a negative control. (b) Immunofluorescence was used to analyze COLEC12 expression in gastric surgical tissue specimens from a gastric cancer patient T21 (Ca) and a non-cancer control individual N1 (N). COLEC12-positive cells are shown in green and those positive for CD90+ stromal cells are shown in red. Isotype control Abs and secondary Abs only were included as negative controls. GSCs N1 derived from an H. pylori-negative, non-cancer control subject was used for reference. The other three paired GSCs of tumor (T2, T9, and T21) and adjacent normal parts (N2, N9, N21) were from gastric cancer patients.