Figure 6.
EGABA is not altered by PNA in GnRH neurons from 3-week-old females. A, Top, Voltage step protocol from −65 to −20 mV. Bottom, Representative gramicidin perforated-patch recording showing the current response to voltage steps. * and # show where measurements were made of baseline and GABAAR-mediated current, respectively. Capacitive transients at the start and end of the variable step protocol were truncated for clarity. B, Measurements of EGABA in control and PNA females. Open and closed circles show recordings using 1 and 140 mm chloride pipette solutions, respectively. Data were analyzed using two-tailed, unpaired Student's t test, p = 0.7048.