Figure 7.

The C-terminal Myc tag on AAV-Grn disrupts sortilin binding. The C-terminal myc-tag on the AAV-Grn almost completely eliminated coimmunoprecipitation of progranulin with sortilin in HEK-293 cells cotransfected with the AAV-Grn construct and a human sortilin plasmid. In contrast, an identical AAV-Grn construct with an HA tag inserted at the N terminus of the protein after the signal peptide exhibited strong coimmunoprecipitation with sortilin. Coimmunoprecipitation data are representative of four experiments.