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. 2018 Feb 28;8:3824. doi: 10.1038/s41598-018-22174-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Validation of inhibition of Ift88 gene expression, N = 4, student’s t-test with Welch’s correction, *p < 0.05. (B) Frequency of primary cilium formation following transfection with siRNA, N = 4–6, n = 286–293, Fisher’s exact test, ***p < 0.001. (C,D) Immunostaining of cells transfected with either off target control (C) or Ift88 targeted siRNA (D) stained with acetylated α-tubulin, (primary cilium, red; arrows) and pericentrin (centrioles, green; arrow head). Nuclei are counterstained with DAPI (blue). Scale bars represent 5μm and 1μm (insert). (E) Cox2 and (F) Opn gene expression in MSCs transfected with siRNA targeting Ift88 or scrambled control, following 2 hours of treatment with 10 nM GSK101 or vehicle control, represented as Mean ± SEM, N = 3, n = 5–8, 2 way ANOVA with Bonferroni post-test, *p < 0.05, ***p < 0.001 significant in comparison to vehicle control, ^$$p < 0.01 significant effect of GSK101 treatment, ^&&&p < 0.001 significant effect of the treatment on the response to GSK101.