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. 2018 Feb 28;8:3769. doi: 10.1038/s41598-018-20459-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Simulated microgravity alters cytoskeleton and inhibits focal adhesions. BL6-10 cells were cultured in chamber slides for 1 day at 1 g, µg and µg + CNF1, and cells were analyzed by light microscopy (A). The cells were also stained with FITC-phalloidin (green) plus PI (red) and FITC-anti-tubulin antibody (green) plus PI (red), respectively (B) or stained with anti-paxillin (green) and anti-vinculin (red) antibody (C) and analyzed by fluorescence microscopy. (D) Paxillin and vinculin spots were counted for each cell. Approximately, 20 cells were analyzed per experimental condition. *p < 0.05 versus indicated groups. One representative experiment of two is shown.