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. 2018 Feb 28;8:3769. doi: 10.1038/s41598-018-20459-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Simulated microgravity inhibits FAK and RhoA activation. (A) Lysates prepared from BL6-10 cells cultured for 3 days at 1 g or µg or µg + CNF1 were subjected to SDS-PAGE analysis. Proteins were transferred onto PVDF membranes and blotted with indicated antibodies. Western blot band signals were quantified by chemiluminescence. Densitometric values were normalized to matching GAPDH controls. Data represent the mean ± SD of three independent experiments. (B) BL6-10 cells exposed for 3 days to 1 g, µg and µg + CNF1 were analyzed for RhoA activity by using the G-LISA RhoA Activation Assay Biochem kit. Data represent the mean ± SD of three independent experiments. **p < 0.05 versus indicated groups. One representative experiment of two is shown.