Table 6.
Growth conditions and Kinetic parameters for biomass producton, nano-MgO production, and glucose consumption during the endophytic S. coelicolor strain E72 cultivation in different cultivation scales and mode of operation in shake flask and in stirred tank bioreactor.
| Parameters | Shake flask | Batch fermentation | Fed- Batch fermentation |
|---|---|---|---|
| Growth conditions | |||
| Container volume (ml) | 1000 | 7000 | 7000 |
| Working Volume (ml) | 500 | 2500 | 5500 |
| pH | Un-controlled | 6.9 | |
| Aeration (v/v) | Un-controlled | Adjusted to let the dissolved O2 level did not below 40% | |
| Agitation (RPM) | 200 | Adjusted to let the dissolved O2 level did not below 40% | |
| Feeding rate (g/l/hr) | 0.42 | ||
| Fermentation period (hr) | 118 | 96 | 192 |
| Yield coefficients | |||
| Yx/s (g/g) | 2.465 | 4.36 | 12.33 |
| Yp/x (g/g) | 0.46 | 0.89 | 1.10 |
| Yp/s (g/g) | 3.595 | 3.9 | 13.53 |
| Growth and glucose consumption parameters | |||
| Xmax-Biomass (g/l) | 24.65 | 43.6 | 123.3 |
| Xmax-time (h) | 96 | 96 | 240 |
| dx/dt (g/l/h) | 0.058 | 0.056 | 0.057 |
| μmax (1/h) | 0.052 | 0.32 | 0.024 |
| Nano-MgO production parameters | |||
| Pmax-vol (g/l) | 35.95 | 39 | 135.3 |
| Pmax-time (h) | 90 | 90 | 246 |