Table 7.

Taguchi’s L₃₂ (2⁴) Orthogonal array design experimental setup for final optimization of nano-MgO biogenic synthesis reaction by using the endophytic S. coelicolor strain E72 intracellular extract.

Exp	Coded levels of independent variables				Nano-MgO (g/l)		S/N ratio [dB]
	Metabolites conc	Mg(NO3)2.6H2O	pH	Temperature	Actual value ± SD	Predicted value
1	1	1	1	1	120 ± 00.00	120.72	41.58
2	1	1	1	1	120 ± 00.00	120.72	41.58
3	1	1	1	2	130 ± 00.00	133.03	42.28
4	1	1	1	2	130 ± 00.01	133.03	42.28
5	1	1	2	1	122 ± 00.00	123.53	41.73
6	1	1	2	1	122 ± 00.01	123.53	41.73
7	1	1	2	2	133 ± 00.00	135.84	42.48
8	1	1	2	2	133 ± 00.01	135.84	42.48
9	1	2	1	1	235 ± 21.21	237.28	47.37
10	1	2	1	1	235 ± 21.22	237.28	47.37
11	1	2	1	2	250 ± 00.00	249.59	47.96
12	1	2	1	2	250 ± 00.01	249.59	47.96
13	1	2	2	1	250 ± 00.00	240.09	47.96
14	1	2	2	1	250 ± 00.01	240.09	47.96
15	1	2	2	2	252.5 ± 03.53	252.41	48.04
16	1	2	2	2	252.5 ± 03.54	252.41	48.04
17	2	1	1	1	185 ± 07.07	184.16	45.33
18	2	1	1	1	185 ± 07.08	184.16	45.33
19	2	1	1	2	205 ± 07.07	196.47	46.23
20	2	1	1	2	205 ± 07.08	196.47	46.23
21	2	1	2	1	185 ± 07.07	186.97	45.33
22	2	1	2	1	185 ± 07.08	186.97	45.33
23	2	1	2	2	200 ± 00.00	199.28	46.02
24	2	1	2	2	200 ± 00.01	199.28	46.02
25	2	2	1	1	300 ± 00.00	300.72	49.54
26	2	2	1	1	300 ± 00.01	300.72	49.54
27	2	2	1	2	310 ± 00.00	313.03	49.83
28	2	2	1	2	310 ± 00.01	313.03	49.83
29	2	2	2	1	300 ± 00.00	303.53	49.54
30	2	2	2	1	300 ± 00.01	303.53	49.54
31	2	2	2	2	315 ± 07.07	315.84	49.96
32	2	2	2	2	315 ± 07.08	315.84	49.96
Level	(v/v)	(M)		(°C)
1	10%	0.5	5	50
2	100%	1	7	100