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. 2017 Nov 21;118(4):546–557. doi: 10.1038/bjc.2017.411

Figure 4.

Figure 4

Both cadherin-1 and cadherin-3 regulate cancer cell invasion. (A) The impact of cadherins expression on cell–cell adhesion properties was assessed by a spheroid formation assay. BxPC-3-cadh-1+/cadh-3+ (no cadherin depletion), BxPC-3-cadh1+ (cadherin-3 depletion) BxPC-3-cadh-3+ (cadherin-1 depletion) cells, as well as primary cultures from human tumours CRCM110 and CRCM08 were allowed to form spheroids in suspension for 24 h. The spheroid area was measured by phase contrast microscopy and analysed by ImageJ. Data represent the mean±s.d. of three separate experiments performed in triplicates. (B) BxPC-3 cell models were allowed to form spheroids for 72 h. Spheroids were then embedded in type I collagen. After the embedding followed by a 24 h incubation period, the spheroid area was measured by phase contrast microscopy and analysed by ImageJ. Data represent the mean±s.d. of three independent experiments performed in triplicates. (C) Isolated BxPC-3 cells and primary cultures from human tumours CRCM110 and CRCM08 were allowed to invade a layer of type I collagen for 24 h. The invasion index was calculated as the mean number of migrated cells counted in 10 microscopic fields. Data represent the mean±s.d. of three independent experiments performed in triplicates. **P<0.01, ***P<0.001.