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. 2017 Nov 21;118(4):546–557. doi: 10.1038/bjc.2017.411

Figure 5.

Figure 5

Cadherin-3 drives migration of pancreatic tumour cells, whereas cadherin-1 is involved in invadopodia formation. (A) BxPC-3 cell monolayers were wounded and incubated in culture medium. Results are expressed as the percentage of wound area closure which was determined after 6 h of incubation. Data represent the mean±s.d. of three independent experiments performed in triplicates. (B) Isolated BxPC-3 cells were plated onto type I collagen. For 6 h the single cell trajectories were analysed by videomicroscopy by capturing images every 5 min. Different parameters such as the speed, the velocity and the directionality were analysed from the cell tracks obtained using ImageJ software. (C) Isolated BxPC-3 cells and primary cultures from human tumours were plated for 16 h on FITC-conjugated gelatin. The areas of degraded matrix were observed with confocal microscope. ImageJ software was used to evaluate the number of invadopodia per cell. Data represent the mean±s.d. of three independent experiments performed in triplicates. **P<0.01, ***P<0.001.