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. 2018 Feb 28;8:3765. doi: 10.1038/s41598-018-21914-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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JMJD-1.2 is expressed in the germline. (a) Representative western blot analysis of lysates extracted from the indicated genotypes using JMJD-1.2 antibody. Actin is used as loading control. (b) Representative images of wild-type (N2) animals (adult, left panel; L1 stage, middle and right panels) stained with JMJD-1.2 specific antibody (lower panels) and DAPI staining (upper panels). a, anterior part of the animals, p, posterior part of the animal. Arrowheads indicate the precursor germ cells at L1 stage. Scale bars, 100 μm (left panel) and 10 μm (middle and right panels). (c) Germline excised from N2 young adult hermaphrodite, reconstructed using ImageJ. The mitotic region is on the left and oocytes are in separate panels on the far right. The top panel shows DAPI staining and the bottom panel anti-JMJD-1.2 staining. 100× magnification; scale bar, 10 μm. MR, mitotic region; TZ, transition zone; PR, pachytene region, DK, oocytes in diakinesis. (d) Relative expression of jmjd-1.2 measured by quantitative PCR using glp-4(bn2), grown at 20 °C (gemline+) and at the restrictive temperature of 25 °C (germline −), in which the gonads are absent. cdc-42 is used as internal control. Bar indicates SD from three independent experiments.