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. 2018 Feb 28;8:3803. doi: 10.1038/s41598-018-22102-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RT-LAMP detection of ZIKV. (A) ZIKA RT-LAMP amplification of ZIKV PCR Standard (ZIKV; Robert Koch Institute) but not no template control (NTC; negative control) as visualized by addition of SYBR Green I (SYBR) by eye (upper panel), green fluorescence (middle panel), or gel electrophoresis (bottom panel). Lane M: Low DNA Mass Marker (ThermoFisher Scientific). (B) All primers (All) are required for effective LAMP reaction. Reactions without FIP and BIP (-FIP/BIP) or FL and BL (-FL/BL) resulted in a negative RT-LAMP reaction.