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. 2018 Feb 28;8:3803. doi: 10.1038/s41598-018-22102-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ZIKV and Actin detection in mosquitos. (A) qRT-PCR of ZIKV RNA normalized to total RNA for mock (n = 5) or ZIKV (n = 5) infected mosquitos. (B,C) Single mosquitos were infected with either mock, ZIKV, or DENV and subjected to a ZIKV (B) or Aedes aegypti Actin (C) specific RT-LAMP reaction then visualized by addition of SYBR Green I by eye (upper panel), green fluorescence (middle panel), or gel electrophoresis (bottom panel). Lane M: Low DNA Mass Marker (ThermoFisher Scientific); NTC: No template control (negative control). Relative density (RD) of the entire bands for a column relative to the DNA Mass Marker are indicated below the corresponding lane. Lanes that did not have any detectable bands over background are reported as not detectable (ND). (D) Detection of ZIKV or ribosomal s17 by qRT-PCR. Data shown as mean ± standard deviation.