Table 2.
RT-LAMP Primer Sequences used to detect ZIKV, human 18 s rRNA, and Ae. aegypti actin.
| Target | Primer | Sequence (5′-3′) |
|---|---|---|
| Homo sapien 18 S rRNA | 18 SrRNA-L3 | GTTCAAAGCAGGCCCGAG |
| 18 SrRNA-B3 | CCTCCGACTTTCGTTCTTGA | |
| 18SrRNA-FIP | TGGCCTCAGTTCCGAAAACCAACCTGGATACCGCAGCTAGG | |
| 18SrRNA-BIP | GGCATTCGTATTGCGCCGCTGGCAAATGCTTTCGCTCTG | |
| 18SrRNA-LF | AGAACCGCGGTCCTATTCCATTATT | |
| 18SrRNA-LB | ATTCCTTGGACCGGCGCAAG | |
| ZIKV | Zika-L3 | GCAGAGCAATGGATGGGATA |
| Zika-B3 | CCCATCCTTGAGGTACAGCT | |
| Zika-FIP | AACCTGAGGGCATGTGCAAACCGCGGTCAGTGGAGATGACT | |
| Zika-BIP | CACAGGAGTGGAAACCCTCGACTGAAGTGGTGGGAGCAGAA | |
| Zika-LF | TCGATTGGCTTCACAACGC | |
| Zika-LB | GGAGCAATTGGGAAGAAGTCC | |
| Ae. aegypti actin | Ae21-F3 | CGGCGCCACCACAAGA |
| Ae21-B3 | TCGTGCCTGTGTTTGTCG | |
| Ae21-LF | ACCGCAAGGCCAAGAACCG | |
| Ae21-FIP | TGCTTGGTCCCTGCCTGGGAGACAGCCCACCAGAACGA | |
| Ae21-BIP | GAGACGAGAACGGCCCAGCGGGTCTGGTGTGTGTCTTTG |