MAD2L2 expression was inversely correlated with NCOA3 expression and predicted favorable prognosis in CRC patients. (A) NCOA3 interaction partners were identified in SW620, HCT116, and HT29 cells stably overexpressing NCOA3 by IP‐MS. (B) The interaction between MAD2L2 and NCOA3 was confirmed by co‐IP in HCT116 cells with stable MAD2L2 overexpression. (C) The basic expression of MAD2L2 and NCOA3 in FHC colon epithelial cells and different CRC cells (SW620, SW480, HCT116, HT29, RKO, and DLD1) and the relative protein level in different CRC cells were shown in C right. (D) Representative images of MAD2L2 and NCOA3 expression in CRC tissues and adjacent normal tissues (ANT). (E) The expression of MAD2L2 and NCOA3 was negatively correlated with CRC tissues. (F) High expression of MAD2L2 was associated with good prognosis in CRC patients. (G) High expression of NCOA3 was related to poor prognosis in CRC patients. (H) High expression of MAD2L2 with low expression of NCOA3 (green curve) predicted favorable prognosis in CRC patients, compared with low expression of MAD2L2 and high expression of NCOA3 (red curve), along with both low and high expression of MAD2L2 and NCOA3 (yellow curve). M means MAD2L2 and N means NCOA3.