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. 2018 Feb 6;12(3):373–390. doi: 10.1002/1878-0261.12171

Figure 6.

Figure 6

AP4 promotes HCC cell migration and invasion via LAPTM4B. (A, B) Knockdown of TFAP4 (LNA‐) in Huh7 cells and BEL‐7402 cells decreased migration (A) and invasion (B), and overexpression of LAPTM4B (L+AN) increased migration and invasion in transwell assays (**P < 0.01). Cell migration and invasion were rescued in the group with AP4 knockdown and LAPTM4B overexpression (L+A−). The cells were fixed after 24 h of incubation. (C) Several important tumorigenic genes were detected by western blot analysis. (D) p‐Myc (Thr58) expression was detected by western blot analysis. (E) Cell lines with different c‐Myc levels were detected by western blot analysis. (F) c‐Myc was knocked by siRNA at a working concentration 50 nm or inhibited by a 48‐h incubation with the c‐Myc inhibitor (10058‐F4) at 100 and 200 μm. (G) TFAP4‐LAPTM4B‐c‐Myc may form a positive feedback regulation loop.