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. 2018 Apr;365(1):60–71. doi: 10.1124/jpet.117.245746

Fig. 3.

Fig. 3.

TLR9 agonist ODN2395-dependent phosphorylation of AMPKα is prevented by inhibiting TAK1 in VSMCs. Protein expression analysis was performed for phospho (p)-AMPKαThr172 normalized to total AMPKα after incubation in either vehicle (Veh) or ODN2395 (2 μmol/l), with and without (57)-7-oxozeaenol (100 nmol/l) (A); phospho (p)-TAK1Thr184/187 normalized to total TAK1 after incubation in either vehicle or ODN2395, with and without ODN2088 (20 μmol/l) (B); phospho-AMPKαThr172 normalized to total AMPKα after incubation in either vehicle or ODN2395, with and without bafilomycin A1 (10 nmol/l) (C); and phospho-LKB1Ser428 normalized to total LKB1 after incubation in either vehicle or ODN2395, with and without (57)-7-oxozeaenol (D). Above, representative images of immunoblots; below, densitometric analysis. n = 5–8. One-way ANOVA, *P < 0.05 vs. vehicle.