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. 2018 Jan 24;12(3):305–321. doi: 10.1002/1878-0261.12162

Figure 5.

Figure 5

Inhibition of Smad3 pathway significantly affects TGF‐β‐induced increase in EGFR expression. (A) Quantitative PCR analysis of the expression level of EGFR mRNA in two breast cancer cells pretreated with SB431542 and then stimulated with TGF‐β for 24 h. Statistical analysis was performed by one‐way ANOVA (*P < 0.05; **P < 0.01 ns: no significant) (B) Western blot analysis of EGFR, total and phosphorylated Smad3, total and phosphorylated ERK protein expression in cell lysates from two breast cancer cells pretreated with SB431542 and then stimulated with TGF‐β for the indicated times. (C) Knockdown of Smad3 weakens TGF‐β‐induced upregulation of EGFR mRNA in breast cancer cells. Statistical analysis was carried out by two‐way ANOVA (***P < 0.001; ****P < 0.0001). (D) Western blot analysis of the effect of Smad3 knockdown on TGF‐β‐induced upregulation of EGFR protein level.