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. 2018 Jan 24;12(3):305–321. doi: 10.1002/1878-0261.12162

Table 2.

Primers used in ChIP assay

Name Primer Sequence Length (bp)
Sp1 (‐234/‐212) Upper 5′ CTGGGAACGCCCCTCTC 3′ 149
Lower 5′ AACCAGCAGCGGGGAC 3′
Sp1 (‐83/‐73)/(‐64/‐52) Upper 5′ CCCGCTGCTGGTTCTCCTCCCTC 3′ 141
Lower 5′ GGCTGCCCGGACGTCTAGCTC 3′
Smad3 (‐1240/‐1231) Upper 5′ TGACTTCAACGCACAGTGGC 3′ 200
Lower 5′ CTTTCCTCCTCATCCAGCAA 3′
Smad3 (‐416/‐407) Upper 5′ CCAGCCTCTGATCCCCGAGA 3′ 148
Lower 5′ TACAAAGCAAACTTGTACCAGC 3′
Positive control (anti‐RNA polymerase II) Upper 5′ TACTAGCGGTTTTACGGGCG 3′ 166
Lower 5′ TCGAACAGGAGGAGCAGAGAGCGA 3′
Negative control Upper 5′ AGGCTGTGAGCTAGAGCCCTAACTG 3′ 289
Lower 5′ AGCACAATACTGGGATGGATTCCAGGGAAC 3′