Table 3. Counterselection Assays Based on Prenyl phosphate-GlcNAc-1-phosphotransferases (WecA vs AglH) and in Vitro Cytotoxicities.
| WecA
inhibition IC50 (μM)a |
|||||
|---|---|---|---|---|---|
| compound | E. coli WecA | M. smegmatis WecA | AglH inhibition IC50 (μM)aM. jannaschii AglH | Vero cells IC50 (μM)a | hemolysis IC50 (μM) sheep blooda |
| UT-17455 (10) | 12.5 ± 6.01 | 12.5 ± 5.42 | 12.5 ± 5.18 | 7.08 ± 5.67 | 70 ± 5.71 |
| UT-17460 (11) | 0.32±4.09 | 0.25±4.84 | 3.61±4.43 | 65±4.65 | 205.8±4.33 |
| tunicamycin | 0.15 ± 7.80 | 0.15 ± 7.22 | 13.27 ± 8.10 | 0.12 ± 7.78 | 15 ± 7.83 |
a
All assay procedures are described in the Supporting Information.