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Fig. 5. Feasibility of combined atomic force and fluorescence microscopy on the biochip. (A) An overlay of the entire field of view of the fluorescence image with the scanned AFM region is shown. The blue circle indicates the position and diameter of the pore underneath the cell layer. (B) The zoom-in of the optical image shows the distribution of ML-GFP on the cells. (C) Vertical deflection image and (D) height image from contact-mode AFM imaging of the same region as in (B).