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. 2018 Jan 11;10(2):568–582. doi: 10.1016/j.stemcr.2017.12.013

Figure 3.

Figure 3

LM-E8 Fragments Maintain Undifferentiated State of Cultured Satellite Cells

(A) Immunostaining of cultured satellite cells by the Matrigel control or the Pre3/4/5-on2 group for anti-PAX7 antibody (green), anti-MYOD antibody (red), and DAPI (blue). Scale bar represents 500 μm.

(B) The relative fluorescence intensity of PAX7 at day 5. The ratio of the Matrigel control was set as 1.0. n = 3 independent experiments. Error bars, SEM; ∗∗p < 0.01.

(C) The percentage of PAX7+MYOD− (blue), PAX7+MYOD+ (pink), and PAX7−MYOD+ (red) cells at day 5. n = 3 independent experiments. Error bars, SEM; ∗p < 0.05, ∗∗p < 0.01.

(D) The relative mRNA levels of Myogenin in satellite cells cultured on the Matrigel control and the Pre3/4/5-on2 condition at day 5. The ratio of the Matrigel control was set as 1.0. n = 3 independent experiments. Error bars, SEM; ∗∗p < 0.01.

(E) Number of PAX7+ satellite cells cultured on the Matrigel control and the Pre3/4/5-on2 group for 5 days. n = 3 independent experiments. Error bars, SEM; ∗p < 0.05.

(F) Ratio of Ki67+ proliferating cells in PAX7+ satellite cells cultured by the Matrigel control and the Pre3/4/5-on2 group for 5 days was calculated. n = 3 independent experiments. Error bars, SEM; ∗p < 0.05.