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. 2018 Jan 11;10(2):568–582. doi: 10.1016/j.stemcr.2017.12.013

Figure 5.

Figure 5

Satellite Cells Cultured by Pre3/4/5-on2 Enhance Regenerative Capacity

(A) EGFP-positive satellite cells were sorted and cultured by the Matrigel control and the Pre3/4/5-on2 group for 5 days. These cells were injected into C57BL/6 tibialis anterior (TA) muscles.

(B) Two weeks after the injection, cross-sections were stained with LMα2 (red) and DAPI (blue). Scale bars represent 100 μm (upper figures) and 500 μm (lower figures).

(C) Number of EGFP-positive fibers in TA muscle was calculated. The ratio of the Matrigel control was set as 1.0. n = 3 independent experiments. Error bars, SEM; ∗∗p < 0.01.