Figure 7.

Stem Cells with High GSH Levels Exhibit Increased Therapeutic Efficacy

(A) Scheme for establishment of the poly(I:C)-induced mouse model of asthma.

(B–F) Naive, FR^High, and FR^Low hES-MSCs, or PBS were administered to mice treated with ovalbumin (OVA) and poly(I:C). (B) H&E staining of lung tissues. Sham, sham-operated. (C) Number of total cells, macrophages, neutrophils, lymphocytes, and eosinophils (n = 30 independent biological replicates from n = 10 mice) and (D) ELISA-based detection of TNF-α, IL-10, and IL-17 in the bronchoalveolar lavage fluid (n = 10 independent biological replicates from n = 10 mice). (E) qPCR analysis of Tnfa, Clcl2, Il1b, Il12a, and Il18 expression using RNA isolated from the lung tissues (n = 10 independent biological replicates from n = 10 mice). (F) Immunofluorescent staining of human β-2 microglobulin expression (hB2M, red) and prosurfactant protein C (SFTPC, green) in the lung tissues. Nuclei were stained with DAPI (blue).

For all bar graphs, values represent mean ± SEM; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001; n.s., not significant. Scale bars, 200 μm (B and F). See also Figure S7.