Figure 2.

Insoluble TDP-43 from the brains of patients serves as a template for the formation of intracellular TDP-43 aggregates in cells expressing a plasmid encoding TDP-43. Comparison of band patterns of Sarkosyl-insoluble fractions from cells treated with insoluble TDP-43 seeds prepared from the brains of patients with type A, B, or C TDP-43 proteinopathy (Mackenzie et al. 2011). A schematic diagram of the banding pattern of TDP-43 COOH-terminal fragments (CTFs) observed from the three different TDP-43 types is also presented. Blots were probed using anti-phosphorylated TDP-43 (pS409/410). Molecular weight markers of migrated protein standards are shown in kDa.