Figure 2.

RNA-Seq Reveals That GFRα1+ Spermatogonia Are Defined by a Transcriptional Signature of Active GDNF and FGF Signaling

(A) Principal-component analysis (PCA) of transcriptomes from five isolated spermatogonial populations from adult and postnatal day 6 (P6) juvenile.

(B) Volcano plot of expression profiles comparing TERT^High GFRα1+ KIT– to TERT^High GFRα1– KIT– cells.

(C) MAP/ERK/protein phosphorylation cluster generated by Cytoscape Enrichment Map of gene set enrichment analysis (GSEA) results for TERT^High GFRα1+.

(D) Cell-cycle/proliferation cluster generated by Cytoscape Enrichment Map of GSEA results for TERT^High GFRα1+.

(E) Indicated cell types were sorted and cytospun. A 2 hr EdU pulse was visualized using Click chemistry, and the cells were then immunostained for the undifferentiated spermatogonia marker PLZF. Scale bar, 25 μm. Percentage of EdU+ cells was quantified. Mean and SEM are shown. (N = 5 mice; N = 900–10,000 cells per condition). ^∗p < 0.05; ^∗∗∗p < 0.001.