Figure 3.

MCAM Is a Cell Surface Marker of the GFRα1+ State

(A) Nine patterns of gene expression changes across adult spermatogonial populations identified as statistically significant by Short Time-series Expression Miner (STEM).

(B) Details on STEM pattern no. 8, containing genes with peak expression in TERT^High GFRα1+ cells, with diminished expression in all other cell types. Genes of interest are highlighted. The entire list of 575 genes is found in Table S2.

(C) Whole-mount analysis of tubules triple-stained for MCAM, GFRα1, and PLZF. All 76/76 GFRα1+ cells were MCAM^High. White arrows point to cells shown in greater magnification in the panels to the right. Scale bar, 50 μm. N = 3 mice.

(D) Flow cytometry measurement of GFRα1 and MCAM expression in TERT^High KIT– cells and TERT^Low KIT+. Panels are representative of at least six independent FACS runs.