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. 2018 Jan 15;32(2):140–155. doi: 10.1101/gad.307884.117

Figure 3.

Figure 3.

Dh31r expression in clock cells is sufficient for TPR. (A–D) Comparison of TPRs during the daytime in RNAi knockdown flies (red line) and control flies (gray line). (A,B) RNAi-mediated knockdown of Dh31r in all neurons using elavG4 (elav-Gal4, a pan-neuronal driver) with Dh31r-RNAi1 (UAS-Dh31r-RNAi1) (A) or Dh31r-RNAi2 (UAS-Dh31r-RNAi2) (B). (C,D) RNAi-mediated knockdown of Dh31r in all clock cells using timG4 (tim-Gal4, an all-clock cell driver) with Dh31r-RNAi1 (UAS-Dh31r-RNAi1) (C) or Dh31r-RNAi2 (UAS-Dh31r-RNAi2) (D). (E) Dh31r expression in all clock cells using tim(UAS)-Gal4 and UAS-Dh31r [tim(UAS)G4>UAS-Dh31r; red line] and the corresponding controls [tim(UAS)G4/+ and UAS-Dh31r/+; gray lines] in the Dh31r2/Df mutant background. The numbers represent the numbers of assays. The results of one-way ANOVA or the Kruskal-Wallis test for the data obtained during the daytime are shown. (**) P < 0.01; (*) P < 0.05, the Tukey-Kramer test or Kruskal-Wallis test compared with ZT1–ZT3 (Supplemental Table S1).