Skip to main content
. 2018 Jan 4;10(2):524–537. doi: 10.1016/j.stemcr.2017.12.003

Figure 4.

Figure 4

IGF-1 Promoted CXCR4 Expression and Cell Migration through HIF-2α Regulation in CD49f+AP+GSCs

(A) Dose effect of IGF-1 (0, 1, 10, and 50 ng/mL) on the morphology of CD49f+AP+GSC cells. (a) Observation of cell adhesion. Scale bar, 100 μm. (b) Quantitative analysis of (a). p < 0.001. One-way ANOVA.

(B) Dose effect of IGF-1 on cell migration. (a) Transwell assay. Scale bar, 100 μm. (b) Quantitative analysis of (a). p < 0.05. One-way ANOVA.

(C) Dose effect of IGF-1 on the protein expression levels of CXCR4, HIF-2α, IGF-1Rβ, and OCT4. (a) Western blotting analysis. β-ACTIN served as an internal control. (b) Quantification of (a) was performed and normalized to the corresponding β-ACTIN. P < 0.0001. Two-way ANOVA.

(D) Effect of HIF-2α knockdown on the protein levels of CXCR4, OCT4, and IGF-1Rβ in CD49f+AP+GSC cells with or without IGF-1 treatment. Western blotting analysis. The relative quantification was normalized to the corresponding β-ACTIN. For all quantifications, data are the means ± SEM of at least three independent experiments. See also Figure S7.