Figure 5.

Inhibition of IGF-1R Activation Suppressed Hypoxia-Induced Cell Migration in Mouse CD49f⁺AP⁺GSCs

(A) Effect of PPP (IGF-1R phosphorylation inhibitor, 1 μM) on hypoxia (1% O₂)-induced SDF-1 expression in CD49f⁺AP⁺GSCs. Immunofluorescence staining. Scale bar, 100 μm.

(B) CXCR4 levels on the surface of CD49f⁺AP⁺GSCs with or without PPP treatment under 1% O₂ hypoxia. (a) Dot blots from the flow cytometry analysis. (b) Percentage of CXCR4-positive cells. Student's t test.

(C) Migration ability of CD49f⁺AP⁺GSCs under different oxygen concentrations with or without PPP treatment. (a) Transwell assay. Scale bar, 100 μm. (b) Quantitative analysis of (a). One-way ANOVA.

(D) Effect of PPP on SDF-1-induced cell migration under 1% O₂ hypoxia. (a) Transwell assay. Scale bar, 100 μm. (b) Quantitative analysis of (a).

(E) Migration ability of CD49f⁺AP⁺GSCs with IGF-1, SDF-1, or PPP treatment for 18 hr under 1% O₂ hypoxia. (a) Wound closure assay. (b) Quantitative analysis of (a). One-way ANOVA. Scale bars, 100 μm.

For all quantifications, data are the means ± SEM of at least three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001. See also Figure S7.