Figure 6.

Crosstalk of IGF-1R and CXCR4 Signaling in Mouse CD49f⁺AP⁺GSCs

(A) Effect of IGF-1 (50 ng/mL), with or without PPP (1 μM) treatment, on the IGF-1R/AKT phosphorylation and CXCR4 transactivation (CXCR4 phosphorylation) of CD49f⁺AP⁺GSCs. (a) Western blot analysis. (b) Quantitative analysis of (a). Student's t test.

(B) Effect of SDF-1 (400 ng/mL), with or without AMD3100 (50 μM) treatment, on the CXCR4/AKT phosphorylation and IGF-1R transactivation (IGF-1R phosphorylation) of CD49f⁺AP⁺GSCs. (a) Western blot analysis. (b) Quantitative analysis of (a). Student’s t test.

(C) Migration ability of CD49f⁺AP⁺GSCs with IGF-1, SDF-1, or PPP treatment for 18 hr under 21% O₂ normoxia. (a) Wound closure assay. Scale bar, 100 μm. (b) Quantitative analysis of (a). One-way ANOVA.

For all quantifications, data are the means ± SEM of at least three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.